CRISPRi chemical genetics and comparative genomics establish genes mediating drug efficiency in Mycobacterium tuberculosis

Defining genetic determinants of drug efficiency with CRISPRi To outline genes that affect drug efficiency in Mtb, we carried out 90 CRISPRi screens throughout 9 medication in H37Rv Mtb. These screens used a genome-scale CRISPRi library15 to allow titratable knockdown for practically all Mtb genes, together with protein coding genes and non-coding RNAs (Fig. 1a). […]